Antibiofilm activity of mushroom extracts against Staphylococcus aureus


  • Anna Čuvalová Institute of Animal Physiology, Centre of Biosciences of the SAS, Soltesovej 4/6, 040 01 Kosice, Slovak Republic
  • Imrich Strapáč Department of Chemistry, Biochemistry and Biophysics, Institute of Pharmaceutical Chemistry, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 041 81 Kosice, Slovak Republic
  • Livia Handrová Institute of Animal Physiology, Centre of Biosciences of the SAS, Soltesovej 4/6, 040 01 Kosice, Slovak Republic
  • Vladimir Kmeť Institute of Animal Physiology, Centre of Biosciences of the SAS, Soltesovej 4/6, 040 01 Kosice, Slovak Republic



biofilm, fungi, ixodid ticks (Acari), milk, Staphylococcus aureus


Mushrooms are a renowned source of products with an array of bioactivities, from antibacterial to antiviral, cytotoxic, antifeeding, antifungal or antioxidant and might be a valuable resource in the search of new bioactive extracts to inhibit biofilm production. We demonstrate the effect of five mushroom water extracts Macrolepiota procera, Pleurotus ostreatus, Auricularia auricula-judae, Armillaria mellea and Laetiporus sulphurous on biofilm formation of four Staphylococcus aureus strains isolated from ixodid ticks (Acari) and ewe´s milk. The PCR was used for detection of virulence genes (hla, isdA, B, bbp, sirB, fnbpA, sdrE, agr II). The ability of biofilm formation and anti-biofilm activity of mushrooms extracts was assessed in a quantitative crystal violet assay. The biofilm formation of S. aureus strains was significantly reduced by all mushrooms extracts (p < 0.001). We showed that more significant anti-biofilm effect of the extracts was of Staphylococcus aureus isolated from ixodid ticks in comparison to Staphylococcus aureus isolated from ewe´s milk. In the present study, A. mellea, P. ostreatus, L. sulphurous, A. auricula-judae and M. procera extracts inhibited biofilm formation by 70.87%, 67.00%, 64.14%, 62.77% and 47.71%, respectively. The results suggest that compounds in mushrooms extracts might be useful to control and handle detrimental infections caused by animal and human pathogens.


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Alves, M.J., Ferreira, I.C.F.R., Lourenço, I., Costa, E., Martins, A., Pintado, M. (2014). Wild mushroom extracts as inhibitors of bacterial Bbiofilm formation. Pathogens, 3, 667–679.

Booth, M.C., Pence L.M., Mahasreshti P., Callegan M.C., Gilmore M.S. (2001). Clonal associations among Staphylococcus aureus isolates from various sites of infection. Infection and Immunity, 69(1), 345–352.

Carvalho, M.P., Türck, P., Abraham, W.R. (2015). Secondary metabolites control the associated bacterial communities of saprophytic Basidiomycotina fungi. Microbes and environments, 30, 196–198.

Cucarella, C., Solano, C., Valle, J., Amorena, B., Lasa, I., Penadés, J.R. (2001). Bap, a Staphylococcus aureus surface protein involved in biofilm formation. Journal of Bacteriology, 183, 2888–2896.

Dale, S.E., Sebulsky, M.T., Heinrichs, D.E. (2004). Involvement of SirABC in iron-siderophore import in Staphylococcus aureus. Journal of Bacteriology, 186(24), 8356–8362.

Jarraud, S., Mougel, C., Thioulouse, J., Lina, G., Meugnier, H., Forey, F., Nesme, X., Etienne, J., Vandenesch, F. (2002). Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease. Infection and immunity, 70(2), 631–641.

Kateete, D.P., Namazzi, S., Okee, M., Okeng, A., Baluku, H., Musisi, N.L., Katabazi, F.A., Joloba, M.L., Ssentongo, R., Najjuka, F.C. (2011). High prevalence of methicillin resistant Staphylococcus aureus in the surgical units of Mulago hospital in Kampala, Uganda. BMC Research Notes, 4(326).

Kostić, M., Smiljković, M., Petrović, J., Glamočlija, J., Barros, L., Ferreira, I.C.F.R., Ćirić, A., Soković, M. (2017). Chemical, nutritive composition and a wide range of bioactive properties of honey mushroom Armillaria mellea (Vahl: Fr.) Kummer. Food and function, 8, 3239–3249.

Li, B., Dong, M. (2010). Inhibition of Escherichia coli biofilm by Auricularia auricula extract. Modern Food Science and Technology, 26, 1067–1070.

Liu, H., Lv, J., Qi, X., Ding, Y., Li, D., Hu, L., Wang, L., Yu, F. (2015). The carriage of the serine-aspartate repeat protein-encoding sdr genes among Staphylococcus aureus lineages. Brazilian Journal of Infectious Diseases, 19, 498–502.

Martín-Rodríguez, A.J., Reyes, F., Martín, J., Pérez-Yépez, J., León-Barrios, M., Couttolenc, A., Espinoza, C., Trigos, A., Martín, V.S., Norte, M., Fernández, J.J. (2014). Inhibition of bacterial quorum sensing by extracts from aquatic fungi: first report from marine endophytes. Marine Drugs, 12, 5503–5526.

Nazzaro, F., Fratianni, F., Coppola, R. (2013). Quorum sensing and phytochemicals. International Journal of Molecular Sciences, 14, 12607–12619.

O’Toole, G.A. (2011). Microtiter dish biofilm formation assay. Journal of Visualized Experiments, 47.

Payne, D.J., Gwynn, M.N., Holmes, D.J., Pompliano, D.L. (2007). Drugs for bad bugs: confronting the challenges of antibacterial discovery. Nature reviews. Drug Discovery, 6(1), 29–40.

Rasmussen, G., Monecke, S., Ehricht, R., Söderquist, B. (2013). Prevalence of clonal complexes and virulence genes among commensal and invasive Staphylococcus aureus isolates in Sweden. PLoS One, 9, (e99097).

Sabat, A., Melles, D.C., Martirosian, G., Grundmann, H., van Belkum, A., Hryniewicz, W. (2006). Distribution of the serine-aspartate repeat protein-encoding sdr genes among nasal-carriage and invasive Staphylococcus aureus strains. Journal of Clinical Microbiology, 44(3), 1135–1138.

Shopsin, B., Mathema, B., Alcabes, P., Said-Salim, B., Lina, G., Matsuka, A., Martinez, J., Kreiswirth, B.N. (2003). Prevalence of agr specificity groups among Staphylococcus aureus strains colonizing children and their guardians. Journal of Clinical Microbiology, 41(1), 456–459.

Strapáč, I., Baranová, M., Smrčová, M., Bedlovičová, Z. (2016). Antioxidant activity of honey mushrooms (Armillaria mellea). Folia Veterinaria, 60(4), 37–41.

Tristan, A., Ying, L., Bes, M., Etienne, J., Vandenesch, F., Lina, G. (2003). Use of multiplex PCR to identify Staphylococcus aureus adhesins involved in human hematogenous infections. Journal of Clinical Microbiology, 41(9), 4465–4467.

Verkaik, N.J., Boelens, H.A., de Vogel, C.P., Tavakol, M., Bode, L.G., Verbrugh, H.A., van Belkum, A., van Wamel, W.J. (2010). Heterogeneity of the humoral immune response following Staphylococcus aureus bacteremia. European Journal of Clinical Microbiology & Infectious Diseases, 29(5), 509–518.

Waryah, C.B., Gogoi-Tiwari, J., Wells, K., Eto, K.Y., Masoumi, E., Costantino, P., Kotiw, M., Mukkur, T. (2016). Diversity of virulence factors associated with West Australian methicillin-sensitive Staphylococcus aureus isolates of human origin. BioMed Research International, 1–10.




How to Cite

Čuvalová , A., Strapáč , I., Handrová, L., & Kmeť , V. (2018). Antibiofilm activity of mushroom extracts against Staphylococcus aureus. Annales Universitatis Paedagogicae Cracoviensis Studia Naturae, 3(2), 17–23.