Dietary supplementation with algae and polyphenols in rabbit male: Effects on semen quality traits
DOI:
https://doi.org/10.24917/25438832.3supp.12Keywords:
extract, rabbit, spermatozoa, mobility, seminal plasma, antioxidantsAbstract
In recent years, many studies have been focused on natural substances that can affect health of animals. A mix of different extracts was used as dietary supplement and it consists exclusively of natural products. Its main components are polyphenols from terrestrial and marine origins and plant polysaccharides. The effect of this supplement on reproduction has not been reviewed in the past what is a reason why its effect on the reproduction potential of male rabbits was tested. The aim of the present study is to determine effects of natural mix during 120-days long in vivo experiment on selected reproductive traits of male rabbits. Natural mix was supplemented in two different concentrations (T1 - 0.3% and T2 - 0.6%) with the basal ingredients of the conventional rabbit feed in pellet form. In our experiments, emphasis was placed on both the spermatozoa concentration and its motility parameters as well as on the properties of seminal plasma and antioxidant activity. The dietary supplementation with the natural extracts mix positively altered the quality traits of rabbit spermatozoa, but these changes were statistically not significant. In experimental T1 group a significant increase of GPx and FRAP content, both regarding the antioxidant markers profile in seminal plasma was recorded. We can conclude that the supplementation of 0.3% of natural mix did not significantly negatively affect any of the studied reproductive parameters of male rabbits, but some improvement in several antioxidant parameters was found.
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Aitken, R.J., Krausz, C. (2001). Oxidative stress, DNA damage and the Y chromosome. Reproduction, 122, 497–506. https://doi.org/10.1530/rep.0.1220497
Benzie, F.F., Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemestry, 239, 70–76. https://doi.org/10.1006/abio.1996.0292
Massanyi, P., Chrenek, P., Lukac, N., Makarevich, A.V., Ostro, A., Zivcak J., Bulla, J. (2008). Comparison of different evaluation chambers for analysis of rabbit spermatozoa motility parameters using CASA system. Slovak Journal of Animal Science, 41(2), 60–66.
Mourvaki, E., Cardinali, R., Dal Bosco, A., Corazzi, L., Castellini, C. (2010). Effects of flaxseed dietary supplementation on sperm qualità and on lipid composition of sperm subfractions and prostatic granules in rabbit. Science Direct, 73, 629–637. https://doi.org/10.1016/j.theriogenology.2009.10.019
Murphy, E.M., Stanton, C., Brien, C.O., Murphy, C., Holden, S., Murphy, R.P., Varley, P., Boland, M.P., Fair, S. (2017). The effect of dietary supplementation of algae rich in docosahexaenoic acid on boar fertility. Theriogenology, 90, 78–87. https://doi.org/10.1016/j.theriogenology.2016.11.008
Okab, A.B, Samara, E.M., Abdoun, K.A., Rafay, J., Ondruska, L., Parkanyi, V., Pivko, J., Ayoub, M.A., Al-Haidary, A.A., Aljumaah, R.S., Massanyi, P., Lukac, N. (2013). Effects of dietary seaweed (Ulva lactuca) supplementation on the reproductive performance of buck and doe rabbits. Journal of Applied Animal Research, 41(3), 347–355. https://doi.org/10.1080/09712119.2013.783479
Rahal, A., Kumar, A., Singh, V., Yadav, B., Tiwari, R., Chakraborty, S., Dhama, K. (2014). Oxidative stress, prooxidants, and antioxidants: The interplay. BioMed Research International, ID 761264, https://doi.org/10.1155/2014/761264
Russo, R., Pucci, L., Giorgetti, L., Árvay, J., Vizzarri, F., Longo, V., Pozzo, L. (2017). Polyphenolic characterisation of plant mixture (Lisosan® Reduction) and its hypocholesterolaemic effect in high fat diet-fed mice. Natural Product Research, 33(5), 651–658. https://doi.org/10.1080/14786419.2017.1402328
SAS user’s guide: Statistics. Version 8 Edition. Cary (2003). NC: SAS Institute.
Shiva, M., Gautam A.K., Verma Y., Shivgotra V., Doshi H., Kumar, S. (2011). Association between sperm quality, oxidative stress, and seminal antioxidant activity. Clinical Biochemistry, 44, 319–324. https://doi.org/10.1016/j.clinbiochem.2010.11.009
Sikiru, A.B., Alemede, I.C., Egena, S.S.A., Ijaiya, A.T. (2018). Oxidative stress and reproductive inefficiencies: The science, evidences, and solutions. Agricultural Extension Journal, 2(1), 17–26.
Yousef, M.I., Abdallah, G.A., Kamel, K.I. (2003). Effect of ascorbic acid and Vitamin E supplementation on semen quality and biochemical parameters of male rabbits. Animal Reproduction Science, 76, 99–111. https://doi.org/10.1016/S0378-4320(02)00226-9